RBS
Part:BBa_K3793001:Design
Designed by: Rupesh Balaji Jayachandran, Enrique de Dios Mateos Group: iGEM21_Aalto-Helsinki (2021-10-21)
Ribosomal Binding site for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We tried to figure out the number of nucleotides to have in between the promoter and this RBS. After miuch consideration and consultation with experts, we fixed upon having anything between zero to four nucleotides.
Source
Elowitz MB, Leibler S. A synthetic oscillatory network of transcriptional regulators. Nature. 2000 Jan 20;403(6767):335-8. doi: 10.1038/35002125. PMID: 10659856.